crisprSQL

Guides

This table shows all guide RNAs with at least two measured off-targets. It can be reordered by clicking on a column title.

Clicking on a guide sequence shows all measured off-targets for the respective guide.

Human

No.	sequence	region	gene ID	assembly	cell line	study	target distribution	measured off-targets
1	AATGACCACTACATCCTCAAGGG	chr1:131892774-131892796		hg19	K562	Cho		5
2	GTCATCTTAGTCATTACCTGAGG	chr1:185056767-185056789	RNF2	hg19	K562	Tsai_circle		34
3	— " —	— " —	RNF2	hg19	U2OS	Tsai_circle		331
4	CTCGTCTGATAAGACAACAGTGG	chr1:198639112-198639134	PTPRC	hg38	HEK293	Cameron		156
5	GCAAAACTCAACCCTACCCCAGG	chr1:198699665-198699687	PTPRC	hg38	HEK293	Cameron		204
6	ATAGGAGAAGATGATGTATAGGG	chr1:207898989-207899011	CD34	hg38	HEK293	Cameron		178
7	GTCATCTTAGTCATTACCTGAGG	chr1:185056773-185056795	RNF2	hg19	HeLa	KimChromatin		3
8	— " —	— " —	RNF2	hg19	HeLa	Kim16		12
9	GGCCTCCCCAAAGCCTGGCCAGG	chr2:73161171-73161193	EMX1	hg19	HEK293	Ran		13
10	GGCCAGGCTTTGGGGAGGCCTGG	chr2:73161174-73161196	EMX1	hg19	HEK293	Ran		17
11	GGCCTCCCCAAAGCCTGGCCAGG	chr2:73161171-73161193	EMX1	hg19	HEK293	Ran		11
12	GGCCAGGCTTTGGGGAGGCCGG	chr2:73161174-73161193	EMX1	hg19	HEK293	Ran		8
13	GAGTCCGAGCAGAAGAAGAAGGG	chr2:73160981-73161004	EMX1	hg19	U2OS	Tsai		27
14	— " —	— " —	EMX1	hg19	U2OS	Tsai		3
15	— " —	— " —	EMX1	hg19	K562	Tsai_circle		157
16	— " —	— " —	EMX1	hg19	U2OS	Tsai_circle		125
17	— " —	— " —	EMX1	hg19	U2OS	Tsai_circle		100
18	GGTGGACAAGCGGCAGATAGCGG	chr2:216109346-216109368	XRCC5	hg38	HEK293	Cameron		178
19	GAGTCCGAGCAGAAGAAGAAGGG	chr2:73160999-73161021	EMX1	hg19	HeLa	KimChromatin		14
20	— " —	— " —	EMX1	hg19	HEK293	Frock		13
21	— " —	— " —	EMX1	hg19	HeLa	Kim16		27
22	— " —	— " —	HCN1	hg19	U2OS	Kleinstiver		6
23	GCAGCCAGTACAGCTCACCAAGG	chr2:113832869-113832891	CSRNP3	hg19	U2OS	Kleinstiver		9
24	GAGTCCGAGCAGAAGAAGAAGGG	chr2:73160982-73161004	EMX1	hg19	U2OS	Listgarten		8
25	GTCACCTCCAATGACTAGGGTGG	chr2:73161066-73161088	EMX1	hg19	U2OS	Listgarten		10
26	GAGTCCGAGCAGAAGAAGAAGGG	chr2:73160997-73161019	EMX1	hg19	HEK293	Slaymaker		4
27	TGACATCAATTATTATACATCGG	chr3:46414423-46414445	RP11-24F11.2	hg19	K562	Cho		13
28	GAATCCTAAAAACTCTGCTTCGG	chr3:46415040-46415062	RP11-24F11.2	hg19	K562	Cho		13
29	GACACCGAAGCAGAGTTTTTAGG	chr3:46415044-46415066	RP11-24F11.2	hg19	K562	Cho		9
30	GGTCCTGCCGCTGCTTGTCATGG	chr3:46415002-46415024	RP11-24F11.2	hg19	K562	Cho		13
31	AAATGAGAAGAAGAGGCACAGGG	chr3:46415068-46415090	RP11-24F11.2	hg19	K562	Cho		13
32	CCTGCCTCCGCTCTACTCACTGG	chr3:46414489-46414511	RP11-24F11.2	hg19	K562	Cho		11
33	ATGAACACCAGTGAGTAGAGCGG	chr3:46414496-46414518	RP11-24F11.2	hg19	K562	Cho		12
34	AACACCAGTGAGTAGAGCGGAGG	chr3:46414493-46414515	RP11-24F11.2	hg19	K562	Cho		6
35	CCAGTGAGTAGAGCGGAGGCAGG	chr3:46414489-46414511	RP11-24F11.2	hg19	K562	Cho		11
36	GAACACAAAGCATAGACTGCGGG	chr5:87240596-87240619		hg19	HEK293	Tsai		2
37	— " —	— " —		hg19	HEK293	Tsai_circle		47
38	— " —	— " —		hg19	K562	Tsai_circle		319
39	— " —	— " —		hg19	HeLa	KimChromatin		2
40	— " —	— " —		hg19	HeLa	Kim16		17
41	GATGATGATGCCCCGGGCGTTGG	chr5:178418448-178418470	GRM6	hg19	U2OS	Kleinstiver		7
42	GGGTGGGGGGAGTTTGCTCCTGG	chr6:43737291-43737313	FOXJ3	hg19	HAP1	Kim		85
43	— " —	— " —	IGDCC3	hg19	U2OS	Tsai		35
44	GACCCCCTCCACCCCGCCTCCGG	chr6:43738555-43738578	PAPD7	hg19	U2OS	Tsai		223
45	GGTGAGTGAGTGTGTGCGTGTGG	chr6:43737453-43737476	MAX	hg19	U2OS	Tsai		83
46	GTGGGGGGAGTTTGCTCCTGG	chr6:43737290-43737311	KRT42P	hg19	U2OS	Tsai		9
47	GTGAGTGAGTGTGTGCGTGTGGG	chr6:43737455-43737477		hg19	U2OS	Tsai		15
48	GACCCCCTCCACCCCGCCTCCGG	chr6:43738556-43738578	VEGFA	hg19	U2OS	Tsai_circle		2213
49	GGTGAGTGAGTGTGTGCGTGTGG	chr6:43737454-43737476		hg19	U2OS	Tsai_circle		1217
50	GGGTGGGGGGAGTTTGCTCCTGG	chr6:43737291-43737313		hg19	U2OS	Tsai_circle		496
51	— " —	— " —		hg19	U2OS	Tsai_circle		407
52	— " —	— " —		hg38	HEK293	Cameron		422
53	GGGTGGGGGGAGTTTGCTCCAGG	chr6:43737297-43737319		hg19	HeLa	KimChromatin		30
54	GGGTGGGGGGAGTTTGCTCCTGG	chr6:43737291-43737313		hg19	HEK293	Frock		38
55	GGGTGGGGGGAGTTTGCTCCAGG	chr6:43737294-43737313		hg19	HeLa	Kim16		36
56	GACCCCCTCCACCCCGCCTCCGG	chr6:43738556-43738578	VEGFA	hg19	HeLa	Kim16		29
57	GGTGAGTGAGTGTGTGCGTGTGG	chr6:43737454-43737476		hg19	HeLa	Kim16		45
58	GATGGTAGATGGAGACTCAGGGG	chr6:41309631-41309653	NCR2	hg19	U2OS	Kleinstiver		19
59	GACCCCCTCCACCCCGCCTCCGG	chr6:43738556-43738578	HDLBP	hg19	U2OS	Listgarten		21
60	GGTGAGTGAGTGTGTGCGTGTGG	chr6:43737454-43737476		hg19	U2OS	Listgarten		32
61	GACCCCCTCCACCCCGCCTCCGG	chr6:43738556-43738578	HDLBP	hg19	U2OS	Listgarten		144
62	— " —	— " —	PAPD7	hg19	U2OS	Chen17		134
63	GGTGAGTGAGTGTGTGCGTGTGG	chr6:43737453-43737476		hg19	U2OS	Chen17		12
64	— " —	— " —		hg19	HEK293	Slaymaker		12
65	— " —	— " —		hg19	HEK293	Slaymaker		12
66	GGGAAAGACCCAGCATCCGTGGG	chr9:110103687-110103710		hg19	HEK293	Tsai		9
67	GGCCCAGACTGAGCACGTGATGG	chr9:110184619-110184642	RP11-363D24.1	hg19	HEK293	Tsai		5
68	GGGAAAGACCCAGCATCCGTGGG	chr9:110103688-110103710	NTF3	hg19	HEK293	Tsai_circle		50
69	GGCCCAGACTGAGCACGTGATGG	chr9:110184620-110184642	RP11-363D24.1	hg19	HEK293	Tsai_circle		113
70	GGGAAAGACCCAGCATCCGTGGG	chr9:110103688-110103710		hg19	K562	Tsai_circle		119
71	GGCCCAGACTGAGCACGTGATGG	chr9:110184620-110184642	RP11-363D24.1	hg19	K562	Tsai_circle		125
72	— " —	— " —	RP11-363D24.1	hg19	HeLa	KimChromatin		5
73	GGGAAAGACCCAGCATCCGTGGG	chr9:110103688-110103710		hg19	HeLa	Kim16		10
74	GGCCCAGACTGAGCACGTGATGG	chr9:110184620-110184642	RP11-363D24.1	hg19	HeLa	Kim16		19
75	GCATACAGTGATTTGATGAAAGG	chr10:87746680-87746702	PAPSS2	hg38	HEK293	Cameron		182
76	CTTGCCCCACAGGGCAGTAACGG	chr11:5248198-5248220	HBB	hg19	HAP1	Kim		75
77	GGAATCCCTTCTGCAGCACCTGG	chr11:22647331-22647354	FANCF	hg19	U2OS	Tsai		17
78	— " —	— " —	FANCF	hg19	K562	Tsai_circle		395
79	— " —	— " —	FANCF	hg19	U2OS	Tsai_circle		65
80	— " —	— " —	FANCF	hg38	HEK293	Cameron		175
81	GCTGATGTAGTCACTCTTGAGGG	chr11:836294-836316	CD151	hg38	HEK293	Cameron		127
82	CTTGCCCCACAGGGCAGTAACGG	chr11:5248215-5248237	HBB	hg19	HeLa	KimChromatin		43
83	GGAATCCCTTCTGCAGCACCTGG	chr11:22647338-22647360	FANCF	hg19	HeLa	KimChromatin		35
84	GCCTCTTTCCCACCCACCTTGGG	chr11:36594861-36594883	RAG1	hg19	HEK293	Frock		33
85	GGAATCCCTTCTGCAGCACCTGG	chr11:22647332-22647354	FANCF	hg19	HeLa	Kim16		15
86	GCTGCTGCTCTGGTTCCTCGTGG	chr11:3685362-3685384	ART1	hg19	U2OS	Kleinstiver		3
87	GGAATCCCTTCTGCAGCACCTGG	chr11:22647332-22647354	FANCF	hg19	U2OS	Listgarten		4
88	GCTGCAGAAGGGATTCCATGAGG	chr11:22647338-22647360	FANCF	hg19	U2OS	Listgarten		25
89	GGCGGCTGCACAACCAGTGGAGG	chr11:22647156-22647178	FANCF	hg19	U2OS	Listgarten		8
90	GCTGCAGAAGGGATTCCATGAGG	chr11:22647337-22647360	FANCF	hg19	U2OS	Chen17		10
91	GCTTGAGACCGCCAGAAGCTCGG	chr11:22647299-22647322	RP11-735B13.1	hg19	U2OS	Chen17		34
92	GTGCGGCAAGAGCTTCAGCCGGG	chr15:85165028-85165050	RP11-182J1.18	hg19	U2OS	Listgarten		8
93	GCTAGAGTCACAAGTCCCACCGG	chr17:39197443-39197465	KRTAP1-1	hg19	U2OS	Kleinstiver		3
94	GGAGTGAGGGAAACGGCCCCAGG	chr19:10244631-10244654	MYH9	hg19	U2OS	Chen17		8
95	GGCACTGCGGCTGGAGGTGGGGG	chr20:31349755-31349778	CDH4	hg19	HEK293	Tsai		133
96	— " —	— " —		hg19	HEK293	Tsai_circle		1043
97	— " —	— " —		hg19	HeLa	Kim16		38
98	GCATTTTCAGGAGGAAGCGATGG	chr21:36421193-36421215	RUNX1	hg19	U2OS	Listgarten		2
99	TGGATGGAGGAATGAGGAGTTGG	chrX:48544569-48544591	WAS	hg19	HEK293	Wang		10
100	GCCGGAGGGGTTTGCACAGAAGG	chrX:104464363-104464385	DYSF	hg19	U2OS	Kleinstiver		7
101	GACGCATAAAGATGAGACGCTGG	n/a				Finkelstein		7053
102	GTGATAAGTGGAATGCCATGTGG	n/a				Finkelstein		7053

Rodents

No.	sequence	region	gene ID	assembly	cell line	study	target distribution	measured off-targets
1	CATGTGATCGCGCTTCTCGTTGG	n/a		mm10	embryo	Anderson		15
2	TCTGCAGTCGACGGTACCGCGGG	n/a		mm10	embryo	Anderson		15
3	TTGATGGCCCATCTACCTCCAGG	chr1:58752658-58752680	Cflar	mm10	embryo	Anderson		15
4	AGGTACCCTGGTCATTCACTTGG	chr1:94052455-94052477	Pdcd1	mm10	embryo	Anderson		11
5	CGCCAGGAACTCACACTTCATGG	chr2:128779864-128779886	Mertk	mm10	embryo	Anderson		15
6	TGGTCATCCGCAAGCGGAAAGTGG	chr2:26359364-26359387	Card9	mm10	embryo	Anderson		5
7	CCGGAATAGCACCGTGCCACAGG	chr2:26360157-26360179	Card9	mm10	embryo	Anderson		11
8	CATTGAATAAGGCGCCCTGCAGG	chr2:26359069-26359091	Card9	mm10	embryo	Anderson		11
9	AAAGATGCCCGCTATACCAACGG	chr3:88629817-88629839	Arhgef2	mm10	embryo	Anderson		11
10	CACCGCAGCCACGCAGAGCAGG	chr4:106136484-106136504	"Pcsk9"	mm9	N2A	Ran		14
11	GGCCGCTGACCACACCTGCCAGG	chr4:106126920-106126942	"Pcsk9"	mm9	N2A	Ran		18
12	AAGGTACACCACGTACCAGTAGG	chr5:3806082-3806104		rn5	embryo	Anderson		12
13	AAAGGTGTGGCGCTGCTGAAGGG	chr6:4753911-4753933	Peg10	mm10	embryo	Anderson		15
14	GGTGGGTATGGGAACACGTTTGG	chr6:4760659-4760681		mm10	embryo	Anderson		15
15	TGGAAAGCAAAATCATGGCTTGG	chr7:48552843-48552865	Mrgprb2	mm10	embryo	Anderson		11
16	AGAGAGACAGCTTGCAAACTAGG	chr8:85071578-85071600		mm10	embryo	Anderson		11
17	ATCATTGTGCAGGCCTGCAGAGG	chr9:5324934-5324956	Casp4	mm10	embryo	Anderson		11
18	GGAGATGTGGATCTCCGCACTGG	chr10:38767111-38767133		rn5	embryo	Anderson		11
19	AGAAGGAGTTCGCCAAAGCCAAGG	chr10:91098294-91098317		rn5	embryo	Anderson		11
20	TGAGGTGAGGCACTTCTAACAGG	chr11:98626203-98626225		mm10	embryo	Anderson		15
21	AGGTAGGGGGTGAGCCGGCTAGG	chr11:119317211-119317233	Card14	mm10	embryo	Anderson		5
22	TGGGATCCGAAGCACTCTTCTGG	chr12:33111533-33111555		rn5	embryo	Anderson		17
23	ACCATGGAAGGGGATAGGCGAGG	chr14:65971305-65971327	Clu	mm10	embryo	Anderson		15
24	TATTGGTGTGGTGCCTAATTTGG	chr14:30006934-30006956	Il17rb	mm10	embryo	Anderson		15
25	ATCTATAATCATGCCTACCGTGG	chr14:30005718-30005740	Actr8	mm10	embryo	Anderson		15
26	GCAGGGTGCGAGTGATCCAAGGG	chr15:63857461-63857483		mm10	embryo	Anderson		15
27	GGGATTAGGCCAGAGAAGAGGGG	chr15:84172740-84172762	Pnpla3	mm10	embryo	Anderson		15
28	ACTAGGCCCCAGTACTTCACTGG	chr15:66906169-66906191	Ccn4	mm10	embryo	Anderson		15
29	TTTGGTGGCTTTCGTCAATGTGG	chr15:77050721-77050743	Apol6	mm10	embryo	Anderson		12
30	TAGAGTCCGGATCTTGTTGGAGG	chr15:5108286-5108308	Card6	mm10	embryo	Anderson		5
31	AAAGAGGAACATCTCTGCAGAGG	chr16:43662258-43662280	Tigit	mm10	embryo	Anderson		15
32	GTTCTGAGCCCTGGTACCCCTGG	chr17:34914321-34914343		mm10	embryo	Anderson		15
33	ACGTCTACTGAAATTGACCCTGG	chr19:56433105-56433127	Casp7	mm10	embryo	Anderson		15