Database for CRISPR/Sp-Cas9 off-target assays

Sp-Cas9 protein bound to a double-stranded DNA (blue) guided by an sgRNA (orange). PDB: 4UN3.

Database statistics

crisprSQL contains 17 studies, totalling 154 guides and 25632 targets, 4947 of which have at least one epigenetic marker attached.

Contribute to database

crisprSQL invites submissions of Cas9 off-target indel frequency data, in order to be included in the online database and the benchmark dataset. Please click here.

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About crisprSQL

crisprSQL is a SQL-based database for CRISPR/Cas9 off-target cleavage assays. It is a one-stop source for epigenetically annotated, base-pair resolved cleavage frequency distributions.

This hand-curated dataset can act as an insight into state-of-the-art technologies driving transgenics, inform guide RNA design for genome engineering, and serve as a shared, transparent basis for modelling off-target DNA cleavage of CRISPR/Cas. Attached gene IDs make the high-resolution data usable for informing knockout screens, functional genomics and transcriptomics research.

How To

Have a look at the included studies or browse through the included guide RNAs. From there, clicking on a guide takes you to its off-target profile, complete with epigenetic annotations.

crisprSQL is developed at Oxford University, 2020 – 2022